A Clinical Questionnaire for Reveal SNP Microarray - POC must be completed.
Preferred nonfixed tissue or products of conception (POC)/placental villus biopsy; formalin-fixed paraffin-embedded tissue; (Samples received in fixative are subject to additional charge for histopathology processing and reporting); unstained tissue slides
>2-4 mm3 tissue
Sterile container containing transport media provided by our laboratory, Ringer's lactate, or Hanks' balanced salt solution
Aseptically obtain a small piece of fetal tissue that does not appear necrotic. If specimen is a POC, placental villi or membranes may be the only fetal-derived tissue available, and an effort should be made to submit these tissues rather than maternal decidua.
Maintain specimen at room temperature.
Causes for Rejection
Quantity not sufficient for analysis; necrotic tissue submitted
Detects chromosomal imbalance that may be associated with fetal loss and is ideal for detection of complete or partial moles
Unable to determine genotypes on some formalin-fixed tissue (blocks/slides), allowing only genomic dosage to be determined. This assay does not detect balanced rearrangements, low-level mosaicism (<10%), or tetraploidy.
Whole genome SNP-based copy-number microarray analysis targeting 2.695 million copy-number and allele-specific genome sites
Coppinger J, Alliman S, Lamb A, et al. Whole-genome microarray analysis in prenatal specimens identifies clinically significant chromosome alterations without increase in results of unclear significance compared to targeted microarray. Prenatal Diagn. 2009 Dec; 29(12):1156-1166.PubMed 19795450