Microdeletions in the azoospermatic factor (AZF) region of the Y chromosome account for 10-15 percent of azoospermia and 5-10 percent of oligospermia in men. Y chromosome microdeletion analysis examines four regions of the Y chromosome – AZFa, AZFb, AZFc and AZFd – to help identify causes of infertility in males.
This test detects microdeletions in three azoospermia factor chromosome regions (Yq11.23) that contain genes with a role in spermatogenesis. Deletions of all or part of these regions, designated AZFa, AZFb, and AZFc, cause variable clinical symptoms in 5% to 13% of infertile men, ranging from azoospermia and Sertoli cell-only syndrome to severe or mild oligospermia.
Lavender-top (EDTA) tube or yellow-top (ACD-A) tube
Causes for Rejection
Frozen specimen; hemolysis; quantity not sufficient for analysis; improper container; unlabeled or mislabeled specimen
Maintain specimen at room temperature.
False-positive and false-negative results may occur for reasons that include genetic variants, blood transfusions, bone marrow transplantation, erroneous representation of family relationships, or contamination of a fetal sample with maternal cells.