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Maternal Serum Alpha-Fetoprotein (MSAFP)

CPT: 82105

Synonyms

AFP, Maternal Serum; Alpha-Fetoprotein (AFP), Maternal Serum for Open Spina Bifida; Maternal Serum α-Fetoprotein; msAFP;

Expected Turnaround Time

2 - 5 days Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Specimen Requirements


Specimen

Whole blood or serum (unhemolyzed)

Volume

Serum: 2-3 mL Whole Blood: 7 mL

Container

Serum: AFP tube Whole Blood: Serum separator tube (SST) or red-top tube

Collection

Whole Blood: allow to clot; centrifuge (15 minutes at 1,500 rpm) to prevent hemolysis.

Storage Instructions

Refrigerate.

Causes for Rejection

Gross hemolysis; gross lipemia; quantity not sufficient for analysis

Test Details


Use

Risk assessment for fetal open neural tube defects. At 15w-23w6d (optimal 16w-18w6d)

Methodology

Immunochemiluminometric assay (ICMA)