Scroll back to top

Reveal® SNP Microarray – POC

CPT: 81229

Special Instructions

A Clinical Questionnaire for Reveal SNP Microarray - POC must be completed.

Expected Turnaround Time

12 - 18 days Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Specimen Requirements


Specimen

Preferred nonfixed tissue or products of conception (POC)/placental villus biopsy; formalin-fixed paraffin-embedded tissue; (Samples received in fixative are subject to additional charge for histopathology processing and reporting); unstained tissue slides

Volume

>2-4 mm3 tissue

Container

Sterile container containing transport media provided by our laboratory, Ringer's lactate, or Hanks' balanced salt solution

Collection

Aseptically obtain a small piece of fetal tissue that does not appear necrotic. If specimen is a POC, placental villi or membranes may be the only fetal-derived tissue available, and an effort should be made to submit these tissues rather than maternal decidua.

Storage Instructions

Maintain specimen at room temperature.

Causes for Rejection

Quantity not sufficient for analysis; necrotic tissue submitted

Test Details


Use

Detects chromosomal imbalance that may be associated with fetal loss and is ideal for detection of complete or partial moles

Limitations

Unable to determine genotypes on some formalin-fixed tissue (blocks/slides), allowing only genomic dosage to be determined. This assay does not detect balanced rearrangements, low-level mosaicism (<10%), or tetraploidy.

Methodology

Whole genome SNP-based copy-number microarray analysis targeting 2.695 million copy-number and allele-specific genome sites

References

Coppinger J, Alliman S, Lamb A, et al. Whole-genome microarray analysis in prenatal specimens identifies clinically significant chromosome alterations without increase in results of unclear significance compared to targeted microarray. Prenatal Diagn. 2009 Dec; 29(12):1156-1166.PubMed 19795450